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Supplements and Cofactors

NAD+

A coenzyme that powers your cells and drops as you age. The shot delivers higher levels than precursor supplements can. Inject slowly so you don’t flush.

Research referenceCommunity compiled
Research & reference information

This profile compiles research and community-sourced reference information for educational purposes. Information may evolve as research and community knowledge develop. It is not individualized medical advice.

Overview

A coenzyme that powers your cells and drops as you age.

The shot delivers higher levels than precursor supplements can.

Inject slowly so you don’t flush.

What It Does

Master cellular energy coenzyme, declines 50% between age 20 and 50.

Injectable achieves levels impossible with precursor supplements.

Coenzyme in 500+ enzyme reactions.

Substrate for sirtuins (epigenetic repair) and PARP (DNA repair).

Powers ATP production.

Reconstitution

Vial SizeBAC Water / SolventConcentration
500 mg+ 2 mL250 mg/mL
1000 mg+ 4 mL250 mg/mL
Interactive tool

Reconstitution Calculator

Enter the vial amount and diluent volume to calculate concentration. If you already have a target amount, you can also convert it to liquid volume and syringe markings. This tool does not recommend a target amount.

Calculated concentration—Enter both values above.
Optional conversion

Enter a target amount you already have and select the syringe capacity.

Calculated draw—Enter concentration inputs and a target amount.

Mathematical reference only. The calculator does not determine an appropriate target amount. Syringe capacity only changes the maximum volume that fits.

Protocol

PhaseFrequencyAmountNotes
Week 1M/W/F25 mgTolerance ramp. Start gentle to assess flushing and chest-pressure tolerance. Inject slowly over 30–60 seconds.
Week 2–3M/W/F50 mgStep up if Week 1 was tolerated. Continue slow injection.
Weeks 4+M/W/F75 mgStep up if Week 2 was tolerated.
AdvancedM/W/F100 mgWorking dose (300 mg/week, aligned with Empower/Olympia/Tailor Made compounded SubQ ceiling). Hold at lower step if headache, chest pressure, or fatigue developed at the prior step.
EscalationM-F (5×/week)75–100 mgOptional escalation after the working dose is well tolerated for at least 4 weeks. 375–500 mg/week, above the standard compounded ceiling but within research-community range. Step back to 3×/week if any new flushing, headache, fatigue, or chest pressure appears.
Off4 weeks–Recovery cycle for sirtuin/PARP balance. Quarterly 2-week breaks acceptable for ongoing chronic use.
Cycle & Timing

8–12 weeks on / 4 weeks off. Max: ongoing with quarterly 2-week breaks. Cycling supports sirtuin/PARP balance.

Timing

3×/week SubQ, slow injection (30–60 sec); AM preferred (alerting).

Evidence Context

human data is oral precursors (NR/NMN) or IV; no SubQ outcomes trial.

Population Considerations

Unisex, dose by protocol, no sex difference. Infuse slowly regardless of sex (fast infusion causes flushing / cramping). Avoid in pregnancy.

Handling & Stacking

Injection site
Outer thigh preferred for volume tolerance.
Storage
Freeze-dried: -4°F. Reconstituted: 36–46°F, best used within ~14 days (structure-based estimate); a firm limit, potency fades before sterility does. One vial at a time.
Side effects
Flushing, stimulation, insomnia if dosed late. Nausea at higher doses.
What to expect
  • Stimulating warmth and energy during/after injection, normal.
  • Cognitive clarity and metabolic improvement over 4–8 weeks.
  • 14-day stability limit after reconstitution.
  • Supply math 500mg → 2 vials | 1000mg → 1 vial (4-wk @ 100 mg × 3/wk)
Pairs well with
MOTS-c (mitochondrial stack); SS-31 (additive mitochondrial protection); 5-Amino-1MQ (NAD-preserving NNMT inhibition); Glutathione (antioxidant); Resveratrol (sirtuin activation)
Avoid
Active migraine (NAD+ infusions can trigger). Severe cardiovascular disease (BP fluctuations)

Safety & Patient Education

Contraindications
Active migraine (NAD+ infusions can trigger), severe cardiovascular disease (BP fluctuations)
Warnings
Stimulating effect can disrupt sleep if dosed late. Inject slowly to avoid acute flushing/chest pressure.
Drug interactions
Antihypertensives and vasoactive medications: NAD+ administration can cause transient BP changes and chest pressure, monitor BP, especially with rapid push or large doses Stimulants (caffeine high-dose, ADHD medications): additive activation; reduce dose or shift timing if combined Sedatives/sleep medications: NAD+ is alerting and counteracts sedation; avoid evening dosing if combining Methylating agents (TMG, methylfolate, methylcobalamin): theoretical complementary pathway support but no human data; some users titrate methyl donors when NAD+ doses are high Chemotherapy: NAD+ supplementation in active cancer treatment is contested, coordinate with oncology before starting
Labs
Compound-specific: Liver enzymes AST/ALT (baseline), monitor closely if >200 mg/day Out of range? Rising ALT/AST = liver stress → cut the dose (watch closely above 200 mg/day).
Stop criteria
Persistent headache, chest pressure during injection
Patient education
Inject slowly over 30–60 seconds. Don’t dose evening if sleep disruption occurs.
References
agelessrx · researchdosing · peptidedosages · PubMed