Overview
TB-500 is a synthetic peptide representing the active biological domain of Thymosin Beta-4, the body's master actin-regulating and tissue repair protein.
What problem it targets: It targets torn muscles, strained ligaments, tendonitis, joint capsule injuries, chronic systemic inflammation, and sluggish full-body healing.
What It Does
• Regulates cellular actin polymerization: Sequesters G-actin, enabling cells to form new cytoskeletons and rapidly migrate directly to sites of tissue damage.
• Promotes widespread angiogenesis: Stimulates the rapid growth of new micro-capillaries into injured areas, restoring blood flow and vital healing factors to oxygen-starved tissue.
• Systemic tissue regeneration: Unlike localized compounds, TB-500 has low molecular weight and circulates throughout the entire body to heal connective tissues, muscles, and skin.
• Realistic expectations: Administered subcutaneously in loading and maintenance protocols. Users report dramatic reductions in post-injury stiffness and accelerated healing over 3 to 6 weeks.
Reconstitution
| Vial size | BAC water or Solvent | Concentration |
|---|---|---|
| 5 mg | + 0.50 mL | 10 mg/mL |
| 10 mg | + 1 mL | 10 mg/mL |
Protocol
| Phase | Frequency | Dose | Notes |
|---|---|---|---|
| Weeks 1–4 | 2×/week | 2.5 | Loading bolus – saturates tissue rapidly. M/Th split (3–4 days apart) standard. Community-consensus loading |
| mg | (PeptideDeck, PeptideDosingProtocols, ThePeptideReport); 5 mg 2×/wk is the upper end some sources use. | ||
| Weeks 5–8 / | Weekly | 2.5 | Maintenance. Sustains tissue levels after loading. Same day each week. |
| Wolverine stack | mg | ||
| Daily (Wolverine) | Daily | 500 | Alternative cadence when paired daily with BPC-157 / Wolverine Blend. BPC-157’s short half-life forces daily injection; TB- |
| mcg | 500 rides along at smaller per-day amounts that approximate the standalone weekly mg. See Wolverine Blend page for the full daily stack protocol. | ||
| Off | 4 weeks | – | Standard washout. Re-cycle if continued tissue work needed. Cycle: 4 weeks loading + 4 weeks maintenance / 4 weeks off (community standard). Daily-microdose alt: 8–10 weeks daily / 2–4 weeks off (Wolverine-aligned). Timing: Daily or M-F, anytime; Wolverine partner By sex: Unisex, same protocol for men and women, no cycle effect. Systemic angiogenic action; theoretical caution with active cancer. Avoid in pregnancy. |
Handling & Stacking
- Freeze-dried: -4°F (years stable).
- Reconstituted: 36–46°F.
- Best used within 28 days, otherwise potency gradually fades over ~6–8 weeks (structure- based estimate, no published data).
- Discard if any cloudiness or colour change.
- Head rush immediately post-injection is normal.
- Systemic anti-inflammatory within days.
- Recovery improvements across all musculoskeletal tissues over 4–8 weeks.
- Supply math 5mg → 6 vials | 10mg → 3 vials (4-wk load 2.5mg×2/wk + 4-wk maint 2.5mg×1/wk = 30 mg)
